To supplement the deficiency of the latitude and longitude existed as location code, such as complex description, non-regional characteristics and complex computation, a globe spatial grid reference system is constructed based on GeoSOT from Peking University. The grid system, built from a perfect quadtree with one degree, one minute and one second grid, could be fit for air-earth joint action. It designs a simple and practical location coding method, which also supports distance simple calculation. It could realize multi-source spatial data integrated retrieval, and develop methods of efficient code operation, framework of spatial computing, and 3D-earth grid system. Globe Spatial Grid Reference System will definitely play an important role in the future of big spatial data applications.